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Cell-Free Gene Expression: Methods and Protocols

Original price was: ₨ 2,100.Current price is: ₨ 1,550.

Cell-Free Gene Expression: Methods and Protocols is a comprehensive laboratory reference designed for molecular biologists, synthetic biologists, biotechnology researchers, biomedical scientists, graduate students, and laboratory professionals. This practical guide presents standardized protocols and modern methodologies for performing cell-free gene expression experiments, enabling rapid protein production and advanced molecular studies without the use of living cells.

The book covers essential topics including cell-free transcription and translation systems, protein synthesis, DNA template preparation, RNA synthesis, cell lysate preparation, recombinant protein expression, enzyme production, metabolic engineering, synthetic biology, CRISPR applications, gene circuit design, protein purification, high-throughput screening, biomolecular engineering, and molecular diagnostics. Each chapter provides detailed experimental procedures, reagent preparation, troubleshooting advice, and expert recommendations to ensure accurate, efficient, and reproducible laboratory results.

The text also explores applications of cell-free systems in drug discovery, vaccine development, biosensor engineering, enzyme optimization, protein engineering, metabolic pathway reconstruction, personalized medicine, and industrial biotechnology. Supported by workflow diagrams, protocol summaries, illustrations, tables, and practical laboratory notes, the book helps researchers optimize experimental workflows while adopting the latest innovations in molecular biology.

Key Features

  • Comprehensive laboratory protocols for cell-free gene expression.
  • Covers transcription, translation, protein synthesis, and recombinant protein production.
  • Includes synthetic biology, CRISPR applications, metabolic engineering, and molecular diagnostics.
  • Features detailed protocols, troubleshooting tips, workflow diagrams, and laboratory guidance.
  • Explores applications in biotechnology, drug discovery, vaccine development, and protein engineering.
  • Ideal for molecular biologists, synthetic biologists, biomedical researchers, and graduate students.
  • Valuable reference for academic laboratories, biotechnology companies, and research institutions.

Order the original Cell-Free Gene Expression: Methods and Protocols today from The Medical Books and accelerate your molecular biology and synthetic biology research with this essential laboratory reference. Shop now and enjoy fast delivery across Pakistan.

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Description

Description

Cell-Free Gene Expression: Methods and Protocols is a practical laboratory guide providing step-by-step protocols for cell-free gene expression, protein synthesis, molecular engineering, and advanced biotechnology research.

The Medical Books

Additional information

Additional information

Dimensions11 × 8.5 × 21 cm
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